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Inducible expression of p21WAF-1/CIP-1/SDI-1 from a promoter conversion retroviral vector.

Authors
  • Mrochen, S
  • Klein, D
  • Nikol, S
  • Smith, J R
  • Salmons, B
  • Günzburg, W H
Type
Published Article
Journal
Journal of molecular medicine (Berlin, Germany)
Publication Date
Jan 01, 1997
Volume
75
Issue
11-12
Pages
820–828
Identifiers
PMID: 9428612
Source
Medline
License
Unknown

Abstract

Constitutive, high-level expression of the potentially therapeutic WAF-1/CIP-1/SDI-1 gene is incompatible with cell growth. A promoter conversion retroviral vector carrying the WAF-1/CIP-1/SDI-1 gene under the transcriptional control of the glucocorticoid inducible promoter of mouse mammary tumor virus was used to infect human bladder carcinoma or feline kidney cells. Reduced cell growth due to a greater proportion of cells being in the G0/G1 phase of the cell cycle was observed when WAF-1/CIP-1/SDI-1 expression was activated by addition of glucocorticoid hormone. This system demonstrates the potential long-term therapeutic use of WAF-1/CIP-1/SDI-1 delivered by retroviral vectors for inhibiting the growth of rapidly proliferating cells. Moreover, the conditional expression of genes such as WAF-1/CIP-1/SDI-1 from such retroviral vectors may facilitate analysis of their function.

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