Affordable Access

Induced synchrony in Cryptococcus neoformans after release from G2-arrest.

Authors
  • Ohkusu, Misako
  • Raclavsky, Vladislav
  • Takeo, Kanji
Type
Published Article
Journal
Antonie van Leeuwenhoek
Publication Date
Jan 01, 2004
Volume
85
Issue
1
Pages
37–44
Identifiers
PMID: 15031662
Source
Medline
License
Unknown

Abstract

Cryptococcus neoformans was grown first to OD 4 under moderate aeration, then diluted 2.5 times with fresh medium, and grown under limited aeration for 5 h. Oxygen concentration decreased from 5-6 mg l(-1) to 1.5 mg l(-1) 1 h after the shift to limited aeration, and remained at a similar level thereafter. In all the eleven strains examined the shift caused unbudded G(2)-arrest in more than half of the cells. In three strains more than 80% of the cells were arrested in unbudded G(2), and, therefore they were selected for synchrony experiments. After being shifted to extensive aeration again, the cells resumed growth by synchronous budding, followed by synchronous nuclear division. This method has turned out to be a good tool to prepare synchronized culture in C. neoformans, especially when a large amount of synchronized cells is needed. This is worthy of attention, since synchronous cultures after release from G(2)-arrest have not been reported yet in any yeast species.

Report this publication

Statistics

Seen <100 times