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Indocyanine green and iohexol loaded hydroxyapatite in poly(L-lactide-co-caprolactone)-based composite for bimodal near-infrared fluorescence- and X-ray-based imaging.

Authors
  • Górecka, Żaneta1, 2
  • Idaszek, Joanna1
  • Heljak, Marcin1
  • Martinez, Diana C1
  • Choińska, Emilia1
  • Kulas, Zbigniew3
  • Święszkowski, Wojciech1
  • 1 Division of Materials Design, Faculty of Materials Science and Engineering, Warsaw University of Technology, Warsaw, Poland. , (Poland)
  • 2 Centre for Advanced Materials and Technologies CEZAMAT, Warsaw University of Technology, Warsaw, Poland. , (Poland)
  • 3 Faculty of Mechanical Engineering, Wroclaw University of Science and Technology, Wroclaw, Poland. , (Poland)
Type
Published Article
Journal
Journal of Biomedical Materials Research Part B Applied Biomaterials
Publisher
Wiley (John Wiley & Sons)
Publication Date
Jan 01, 2024
Volume
112
Issue
1
Identifiers
DOI: 10.1002/jbm.b.35313
PMID: 37596854
Source
Medline
Keywords
Language
English
License
Unknown

Abstract

This study aimed to develop material for multimodal imaging by means of X-ray and near-infrared containing FDA- and EMA-approved iohexol and indocyanine green (ICG). The mentioned contrast agents (CAs) are hydrophilic and amphiphilic, respectively, which creates difficulties in fabrication of functional polymeric composites for fiducial markers (FMs) with usage thereof. Therefore, this study exploited for the first time the possibility of enhancing the radiopacity and introduction of the NIR fluorescence of FMs by adsorption of the CAs on hydroxyapatite (HAp) nanoparticles. The particles were embedded in the poly(L-lactide-co-caprolactone) (P[LAcoCL]) matrix resulting in the composite material for bimodal near-infrared fluorescence- and X-ray-based imaging. The applied method of material preparation provided homogenous distribution of both CAs with high iohexol loading efficiency and improved fluorescence signal due to hindered ICG aggregation. The material possessed profound contrasting properties for both imaging modalities. Its stability was evaluated during in vitro experiments in phosphate-buffered saline (PBS) and foetal bovine serum (FBS) solutions. The addition of HAp nanoparticles had significant effect on the fluorescence signal. The X-ray radiopacity was stable within minimum 11 weeks, even though the addition of ICG contributed to a faster release of iohexol. The stiffness of the material was not affected by iohexol or ICG, but incorporation of HAp nanoparticles elevated the values of bending modulus by approximately 70%. Moreover, the performed cell study revealed that all tested materials were not cytotoxic. Thus, the developed material can be successfully used for fabrication of FMs. © 2023 Wiley Periodicals LLC.

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