Affordable Access

Indirect immunofluorescence technique to detect monoclonal antinuclear antibody.

Authors
  • Johnson, T L
  • Keren, D F
  • Thomas, L R
Type
Published Article
Journal
Archives of pathology & laboratory medicine
Publication Date
Jun 01, 1987
Volume
111
Issue
6
Pages
560–562
Identifiers
PMID: 3107515
Source
Medline
License
Unknown

Abstract

Monoclonal proteins directed against nuclear antigens are extremely rare. We describe the use of a double antibody immunofluorescence technique to document the existence of a monoclonal antibody with nuclear antigen activity. The patient was an elderly woman with a small monoclonal protein, but no evidence of a lymphoproliferative or autoimmune disorder. A homogeneous antinuclear antibody (ANA) prompted further studies to determine whether the monoclonal protein was related to the ANA activity. A double-antibody immunofluorescence procedure is described by which the ANA positivity was shown to be entirely due to a monoclonal IgMK protein. It consists of using fluorescein anti-kappa and rhodamine anti-lambda applied to a section of mouse kidney treated with the patient's serum. Documentation of antigenic specificity of monoclonal antibodies by double antibody fluorescence provides a straightforward method that can be utilized by routine clinical laboratories.

Report this publication

Statistics

Seen <100 times