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In-Depth Proteomic Analysis of Human Bronchoalveolar Lavage Fluid toward the Biomarker Discovery for Lung Cancers.

Authors
  • Sim, Seo Young1
  • Choi, Yu Ri1
  • Lee, Jun Hyung1, 2
  • Lim, Jae Min1
  • Lee, Seung-Eun3
  • Kim, Kwang Pyo1, 3
  • Kim, Jin Young4
  • Lee, Seung Hyeun5
  • Kim, Min-Sik1, 2, 3
  • 1 Department of Applied Chemistry, Global Center for Pharmaceutical Ingredient Materials, Institute of Natural Science, Kyung Hee University, Yongin, 17104, Republic of Korea. , (North Korea)
  • 2 Department of New Biology, DGIST, Daegu, 42988, Republic of Korea. , (North Korea)
  • 3 Department of Biomedical Science and Technology, Kyung Hee Medical Science Research Institute, Kyung Hee University, Seoul, 02447, Republic of Korea. , (North Korea)
  • 4 Biomedical Omic Research Group, Korea Basic Science Institute, Ochang, 28119, Republic of Korea. , (North Korea)
  • 5 Department of Internal Medicine, Kyung Hee University School of Medicine, Seoul, 02447, Republic of Korea. , (North Korea)
Type
Published Article
Journal
PROTEOMICS - CLINICAL APPLICATIONS
Publisher
Wiley (John Wiley & Sons)
Publication Date
Sep 01, 2019
Volume
13
Issue
5
Identifiers
DOI: 10.1002/prca.201900028
PMID: 31119868
Source
Medline
Keywords
Language
English
License
Unknown

Abstract

Lung cancer is among the most common cancers. Bronchoalveolar lavage fluid (BALF) can be easily obtained from patients with lung cancers. The aim is to develop a novel proteomic method of the molecule-based sensitive detection of biomarkers from BALF. BALF samples are collected from segmental bronchus of 14 patients with lung cancers from Kyung Hee University Hospital. First, BALF proteome is depleted using a depletion column, and then peptides are prepared from the enriched low abundant proteins and fractionated by high pH reverse phase liquid chromatography prior to LC-MS/MS. Data are available via ProteomeXchange with identifier PXD012645. A novel method is developed for in-depth proteomic analysis of BALF by combining antibody-based depletion of high abundant proteins from BALF with high pH peptide fractionation. Peptides are analyzed on a high resolution Orbitrap Fusion mass spectrometer. MaxQuant search result in the identification of 4615 protein groups mapped to 4534 genes. It is found that the method outperformed conventional BALF proteomic methods and it is believed that this method will facilitate the biomarker research for lung cancer. In addition, it is shown that BALF will be a great source of biomarkers of lung diseases. © 2019 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

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