Affordable Access

Access to the full text

Increased Osteogenic Potential of Pre-Osteoblasts on Three-Dimensional Printed Scaffolds Compared to Porous Scaffolds for Bone Regeneration

  • Zamani, Yasaman1, 2
  • Amoabediny, Ghassem2, 3, 4
  • Mohammadi, Javad1
  • Zandieh-Doulabi, Behrouz5
  • Klein-Nulend, Jenneke5
  • Helder, Marco N.4
  • 1 Department of Biomedical Engineering, Faculty of New Sciences and Technologies, University of Tehran, Tehran, Iran;
  • 2 Department of Biomedical Engineering, Research Center for New Technologies in Life Science Engineering, University of Tehran, Tehran, Iran;
  • 3 School of Chemical Engineering, College of Engineering, University of Tehran, Tehran, Iran;
  • 4 Department of Oral and Maxillofacial Surgery/Oral Pathology, Amsterdam University Medical Centers-location VUmc and Academic Centre for Dentistry Amsterdam (ACTA), Amsterdam Movement Sciences, Amsterdam, the Netherlands;
  • 5 Department of Oral Cell Biology, Academic Centre for Dentistry Amsterdam (ACTA)-University of Amsterdam and Vrije Universiteit Amsterdam, Amsterdam Movement Sciences, Amsterdam, the Netherlands
Published Article
Iranian Biomedical Journal
Pasteur Institute of Iran
Publication Date
Jan 13, 2021
DOI: 10.29252/ibj.25.2.78
PMID: 33461289
PMCID: PMC7921523
PubMed Central
  • Full Length


Background: One of the main challenges with conventional scaffold fabrication methods is the inability to control scaffold architecture. Recently, scaffolds with controlled shape and architecture have been fabricated using 3D-printing. Herein, we aimed to determine whether the much tighter control of microstructure of 3DP PLGA/β-TCP scaffolds is more effective in promoting osteogenesis than porous scaffolds produced by solvent casting/porogen leaching. Methods: Physical and mechanical properties of porous and 3DP scaffolds were studied. The response of pre-osteoblasts to the scaffolds was analyzed after 14 days. Results: The 3DP scaffolds had a smoother surface (Ra: 22 ± 3 µm) relative to the highly rough surface of porous scaffolds (Ra: 110 ± 15 µm). Water contact angle was 112 ± 4° on porous and 76 ± 6° on 3DP scaffolds. Porous and 3DP scaffolds had the pore size of 408 ± 90 and 315 ± 17 µm and porosity of 85 ± 5% and 39 ± 7%, respectively. Compressive strength of 3DP scaffolds (4.0 ± 0.3 MPa) was higher than porous scaffolds (1.7 ± 0.2 MPa). Collagenous matrix deposition was similar on both scaffolds. Cells proliferated from day 1 to day 14 by fourfold in porous and by 3.8-fold in 3DP scaffolds. ALP activity was 21-fold higher in 3DP scaffolds than porous scaffolds. Conclusion: The 3DP scaffolds show enhanced mechanical properties and ALP activity compared to porous scaffolds in vitro , suggesting that 3DP PLGA/β-TCP scaffolds are possibly more favorable for bone formation.

Report this publication


Seen <100 times