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Increased Calcitonin Gene-Related Peptide and Macrophages Are Involved in Astragalus membranaceus-Mediated Peripheral Nerve Regeneration in Rats.

Authors
  • Chen, Chung-Chia1, 2
  • Chang, Ling-Chuan3
  • Yao, Chun-Hsu4, 5
  • Hsu, Yuan-Man2
  • Lin, Jia-Horng6
  • Yang, Tse-Yen4
  • Chen, Yung-Hsiang2, 5
  • Chen, Yueh-Sheng2, 4, 5
  • 1 * Linsen Chinese Medicine and Kunming Branch, Taipei City Hospital, Taipei, Taiwan. , (Taiwan)
  • 2 † Graduate Institute of Biomedical Sciences, School of Chinese Medicine, Department of Biological Science and Technology, Graduate Institute of Integrated Medicine, China Medical University, Taichung, Taiwan. , (China)
  • 3 ‡ Department of Radiation Oncology, Taipei Medical University Hospital, Taipei, Taiwan. , (Taiwan)
  • 4 § Biomaterials Translational Research Center, Department of Medical Research, China Medical University Hospital, Taichung, Taiwan. , (China)
  • 5 ¶ Department of Bioinformatics and Medical Engineering, Department of Psychology, College of Medical and Health Science, Asia University, Taichung, Taiwan. , (Taiwan)
  • 6 ∥ Department of Fiber and Composite Materials, Feng Chia University, Taichung, Taiwan. , (Taiwan)
Type
Published Article
Journal
The American journal of Chinese medicine
Publication Date
Jan 01, 2018
Volume
46
Issue
1
Pages
69–86
Identifiers
DOI: 10.1142/S0192415X18500040
PMID: 29298514
Source
Medline
Keywords
Language
English
License
Unknown

Abstract

Astragalus membranaceus (AM) is one of 50 fundamental herbs in traditional Chinese medicine. Previous studies have shown that AM extract can be a potential nerve growth-promoting factor, being beneficial for the growth of peripheral nerve axons. We further investigated the effects of AM extract on regeneration in a rat sciatic nerve transection model. Rats were divided into three groups ([Formula: see text]): normal saline (intraperitoneal) as the control, and 1.5[Formula: see text]g/kg or 3.0[Formula: see text]g/kg of AM extract (every other day for four weeks), respectively. We evaluated neuronal electrophysiology, neuronal connectivity, macrophage infiltration, expression levels and location of calcitonin gene-related peptide (CGRP), and expression levels of both nerve growth factors (NGFs) and immunoregulatory factors. In the high-dose AM group, neuronal electrophysiological function (measured by nerve conductive velocity and its latency) was significantly improved ([Formula: see text]). Expression levels of CGRP and macrophage density were also drastically enhanced ([Formula: see text]). Expression levels of fibroblast growth factor (FGF), NGF, platelet-derived growth factor (PDGF), transforming growth factor-[Formula: see text], interleukin-1 (IL-1), and interferon (IFN)-[Formula: see text] were reduced in the high-dose AM group ([Formula: see text]), while FGF, NGF, PDGF, IL-1, and IFN-[Formula: see text] were increased in the low-dose AM group ([Formula: see text]). These results suggest that AM can modulate local inflammatory conditions, enhance nerve regeneration, and potentially increase recovery of a severe peripheral nerve injury.

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