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Increase in the current variance in bilayer lipid membranes near phase transition as a result of the occurrence of hydrophobic defects.

Authors
  • Anosov, A A1
  • Smirnova, E Yu2
  • Sharakshane, A A3
  • Nikolayeva, E A2
  • Zhdankina, Yu S2
  • 1 I.M. Sechenov First Moscow State Medical University (Sechenov University), 2-4 Bolshaya Pirogovskaya st., 119435 Moscow, Russia; Kotel'nikov Institute of Radio Engineering and Electronics of RAS, Mokhovaya 11-7, Moscow 125009, Russia. Electronic address: [email protected]
  • 2 I.M. Sechenov First Moscow State Medical University (Sechenov University), 2-4 Bolshaya Pirogovskaya st., 119435 Moscow, Russia.
  • 3 Kotel'nikov Institute of Radio Engineering and Electronics of RAS, Mokhovaya 11-7, Moscow 125009, Russia.
Type
Published Article
Journal
Biochimica et biophysica acta. Biomembranes
Publication Date
Dec 06, 2019
Volume
1862
Issue
2
Pages
183147–183147
Identifiers
DOI: 10.1016/j.bbamem.2019.183147
PMID: 31812627
Source
Medline
Keywords
Language
English
License
Unknown

Abstract

Most researchers associate the increase in the permeability of lipid bilayers of artificial and biological membranes observed in various experiments with the formation of hypothetical hydrophobic and hydrophilic pores. Although the existence of hydrophobic defects, as the first stage of the formation of a hydrophilic pore, was hypothesized decades ago from electroporation experiments, the difficulty of describing this stage is determined by the lack of experimental data confirming the existence or at least associated with hydrophobic pores. We explored the increase in the current variance through the lipid membrane, observed when approaching the phase transition from the side of high temperatures, and have associated it with capacitive currents arising in response to the formation of hydrophobic pores. Assuming that the number of hydrophobic pores in a membrane follows a Poisson distribution, and thus, the mean number of hydrophobic pores is equal to the variance of that number, we used the measurements of the membrane current variance to evaluate the number of hydrophobic pores. Analysis of experimental data within this model allows us to estimate the number of hydrophobic pores at or above the phase transition and shows that the number of hydrophobic pores in a membrane close to the phase transition increased 20 times compared to the number of hydrophobic pores existing in the membrane far from the melting transition. Copyright © 2019. Published by Elsevier B.V.

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