Affordable Access

Incorporation and asymmetric orientation of glycophorin in reconstituted protein-containing vesicles.

Authors
  • van Zoelen, E J
  • Verkleij, A J
  • Zwaal, R F
  • van Deenen, L L
Type
Published Article
Journal
European journal of biochemistry / FEBS
Publication Date
May 16, 1978
Volume
86
Issue
2
Pages
539–546
Identifiers
PMID: 658056
Source
Medline
License
Unknown

Abstract

Glycophorin can be incorporated in lipid vesicles without the use of detergents [MacDonald, R.J. and MacDonald, R.C. (1975) J. Biol. Chem. 250, 9206-9214]. In this paper it has been shown that the protein spans the lipid bilayer in these vesicles. In addition it has been shown that the protein is oriented in an asymmetric way with 75-80% of its sugar residues directed to the outside of the vesicles. Freeze-fracturing electron microscopy suggests that the protein is present in a slightly aggregated form. Sonication of these recombinants leads to the formation of small glycophorin-containing vesicles, in which almost all sugar residues are directed to the outside.

Report this publication

Statistics

Seen <100 times