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Incidence and molecular diversity of poleroviruses infecting cucurbit crops and weed plants in Thailand

Authors
  • Cheewachaiwit, S.1, 2, 3
  • Warin, N.4
  • Phuangrat, B.4
  • Rukpratanporn, S.4
  • Gajanandana, O.4
  • Balatero, C. H.3
  • Chatchawankanphanich, O.4
  • 1 Kasetsart University, Center for Agricultural Biotechnology, Kamphaeng Saen Campus, Nakhon Pathom, 73140, Thailand , Nakhon Pathom (Thailand)
  • 2 Center of Excellence on Agricultural Biotechnology: (AG-BIO/PERDO-CHE), Bangkok, 10900, Thailand , Bangkok (Thailand)
  • 3 Hortigenetics Research (S.E. Asia) Limited East-West Seeds, Plant Pathology Department, Chiang Mai, 50290, Thailand , Chiang Mai (Thailand)
  • 4 National Science and Technology Development Agency, National Center for Genetic Engineering and Biotechnology, Pathum Thani, 12120, Thailand , Pathum Thani (Thailand)
Type
Published Article
Journal
Archives of Virology
Publisher
Springer-Verlag
Publication Date
Mar 28, 2017
Volume
162
Issue
7
Pages
2083–2090
Identifiers
DOI: 10.1007/s00705-017-3332-2
Source
Springer Nature
Keywords
License
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Abstract

Overall, 244 samples of cucurbit crops with yellowing symptoms and selected weed species, from 15 provinces in Thailand, were screened by RT-PCR using primers Polero-CP-F and Polero-CP-R. A total of 160 samples (~66%) were infected by poleroviruses. Analysis of a 1.4 kb region covering the 3’ RNA-dependent RNA polymerase (RdRp) gene, the intergenic non-coding region (iNCR), and the coat protein (CP), showed that four poleroviruses, namely, cucurbit aphid-borne yellows virus (CABYV), luffa aphid-borne yellows virus (LABYV), melon aphid-borne yellows virus (MABYV) and suakwa aphid-borne yellows virus (SABYV) were associated with the yellowing symptoms in cucurbit crops. Further analyses indicated presence of putative recombinant viruses referred to as CABYV-R and SABYV-R. CABYV-R was derived from the recombination between MABYV and the common strain of CABYV (CABYV-C). SABYV-R was derived from the recombination of MABYV and SABYV.

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