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Inactivation kinetics of sensory neuron sodium channels depend on the type of hydrogen ion buffer

Authors
  • Krylov, B. V.
  • Podzorova, S. A.
  • Vilin, Yu. Yu.
Type
Published Article
Journal
Neuroscience and Behavioral Physiology
Publisher
Kluwer Academic Publishers-Plenum Publishers
Publication Date
Jan 01, 1998
Volume
28
Issue
1
Pages
65–71
Identifiers
DOI: 10.1007/BF02461914
Source
Springer Nature
Keywords
License
Yellow

Abstract

Tetrodotoxin-sensitive and tetrodotoxin-resistant sodium currents were studied in rat dorsal root ganglion neurons using a patch-clamp method. The type of hydrogen ion buffer used was found to affect the kinetics of the inactivation process. Tris ions irreversibly bound to the inactivation gating apparatus, accelerating the decay in the trailing front of the ion current. The characteristics of this process were clearly nonlinear in this buffer. In HEPES buffer, the inactivation dynamics were slowed for both types of channel studied. Unlike results obtained with tris buffer, there were no sharp changes in the characteristics as compared to those obtained immediately after puncture of the cell membrane. The advantages of using HEPES buffer for studies of the inactivation gating processes of sodium channels are discussed.

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