Affordable Access

Access to the full text

Improving the catalytic characteristics of phenolic acid decarboxylase from Bacillus amyloliquefaciens by the engineering of N-terminus and C-terminus

Authors
  • Li, Qin1, 2
  • Xia, Ying1
  • Zhao, Ting1
  • Gong, Yuanyuan1
  • Fang, Shangling1
  • Chen, Maobin1
  • 1 Hubei Key Laboratory of Industrial Microbiology, School of Food and Biological Engineering, Hubei University of Technology, No.28, Nanli Road, Wuhan, 430068, China , Wuhan (China)
  • 2 Sichuan Agricultural University, No. 46, Xinkang Road, Yaan, 625014, China , Yaan (China)
Type
Published Article
Journal
BMC Biotechnology
Publisher
Springer (Biomed Central Ltd.)
Publication Date
Jul 26, 2021
Volume
21
Issue
1
Identifiers
DOI: 10.1186/s12896-021-00705-7
Source
Springer Nature
Keywords
Disciplines
  • Research
License
Green

Abstract

Background4-vinylphenols produced by phenolic acid degradation catalyzed by phenolic acid decarboxylase can be used in food additives as well as flavor and fragrance industry. Improving the catalytic characters of phenolic acid decarboxylase is of great significance to enhance its practical application.ResultsA phenolic acid decarboxylase (P-WT) was created from Bacillus amyloliquefaciens ZJH-01. Mutants such as P-C, P-N, P-m1, P-m2, P-Nm1, and P-Nm2 were constructed by site-directed mutagenesis of P-WT. P-C showed better substrate affinities and higher turnover rates than P-WT for p-coumaric acid, ferulic acid, and sinapic acid; however, P-N had reduced affinity toward p-coumaric acid. The extension of the C-terminus increased its acid resistance, whereas the extension of the N-terminus contributed to the alkali resistance and heat resistance. The affinity of P-m1 to four substrates and that of P-m2 to p-coumaric acid and ferulic acid were greatly improved. However, the affinity of P-Nm2 to four phenolic acids was greatly reduced. The residual enzyme activities of P-Nm1 and P-Nm2 considerably improved compared with those of P-m1 and P-m2 after incubation at 50 °C for 60 min.ConclusionsThe extension of the N-terminus may be more conducive to the combination of the binding cavity with the substrate in an alkaline environment and may make its structure more stable.

Report this publication

Statistics

Seen <100 times