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Improvement of sperm cryo-survival of cynomolgus macaque (Macaca fascicularis) by commercial egg-yolk-free freezing medium with type III antifreeze protein.

Authors
  • Wang, Shengnan1
  • Duan, Yanchao1
  • Yan, Yaping1
  • Adar, Chen2
  • Braslavsky, Ido2
  • Chen, Bingbing1
  • Huang, Tianzhuang1
  • Qiu, Shuai1
  • Li, Xi1
  • Inglis, Briauna Marie1
  • Ji, Weizhi1
  • Si, Wei3
  • 1 Yunnan Key Laboratory of Primate Biomedical Research, Institute of Primate Translational Medicine, Kunming University of Science and Technology, Kunming 650500, Yunnan, China. , (China)
  • 2 Institute of Biochemistry, Food Science, and Nutrition, Robert H. Smith Faculty of Agriculture, Food, and Environment, & Center for Nanoscience and Nanotechnology, The Hebrew University of Jerusalem, Rehovot 7610001, Israel. , (Israel)
  • 3 Yunnan Key Laboratory of Primate Biomedical Research, Institute of Primate Translational Medicine, Kunming University of Science and Technology, Kunming 650500, Yunnan, China. Electronic address: [email protected] , (China)
Type
Published Article
Journal
Animal reproduction science
Publication Date
Nov 01, 2019
Volume
210
Pages
106177–106177
Identifiers
DOI: 10.1016/j.anireprosci.2019.106177
PMID: 31635783
Source
Medline
Keywords
Language
English
License
Unknown

Abstract

When nonhuman primate sperm undergoes cryopreservation in an egg yolk medium there is an increased risk that the egg yolk might adversely affect the sperm due to containing of avian pathogens. Although commercial egg-yolk-free medium for human sperm cryopreservation has been used for macaque sperm, the cryo-survival remains less than optimal. The present study, therefore, was conducted to determine the optimal concentration of antifreeze protein (AFP) III supplemented in a commercial egg-yolk-free medium for cynomolgus macaque (Macaca fascicularis) sperm cryo-survival. The function of frozen-thawed sperm was evaluated by post-thaw sperm motility, acrosome integrity, and mitochondrial function. Results indicate that the sperm motilities were greater when 0.1, 1, and 10 μg/ml of AFP III were supplemented into the sperm freezing medium (P < 0.05). In addition, the mitochondrial membrane potential was greater in the sperm cryopreserved with the medium that was supplemented with 0.1 μg/ml of AFP III (P < 0.05). The addition of AFP III at any of the concentrations, however, did not have any cryoprotection effect on the sperm acrosome, and the greatest concentrations of AFP III at 100 and 200 μg/ml had detrimental effects on acrosomal integrity (P < 0.05). Results of the present study indicated the methods used are effective for the cryopreservation of cynomolgus monkey sperm while reducing associated health risks due to avian pathogens being present in egg yolk-based extenders. Copyright © 2019 Elsevier B.V. All rights reserved.

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