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An improved technique for selective silver staining of nucleolar organizer regions in human chromosomes.

Authors
Type
Published Article
Journal
Human Genetics
0340-6717
Publisher
Springer-Verlag
Publication Date
Volume
34
Issue
2
Pages
199–206
Identifiers
PMID: 63440
Source
Medline

Abstract

A reliable technique for staining human chromosomal nucleolar organizers (NOR's) with silver solutions is described. The NOR's can be selectively stained dark brown by silver solutions leaving the chromosome arms unstained and available for counterstaining with orcein or Giemsa dyes. Unequivocal identification of chromosome pairs bearing NOR's can be achieved using fluorescent banding techniques followed by silver staining. The silver staining procedure for NOR's was simplified and standardized through control of the chemical and physical conditions during silver impregnation and developing.

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