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Improved streptococcal grouping antisera containing polyethylene glycol.

Authors
  • George, J R
  • Ashworth, H
  • Facklam, R R
  • Harrell, W K
  • Palmer, D F
Type
Published Article
Journal
Journal of clinical microbiology
Publication Date
Oct 01, 1981
Volume
14
Issue
4
Pages
433–436
Identifiers
PMID: 7026609
Source
Medline
License
Unknown

Abstract

Antisera to streptococcal groups A through G containing 4% polyethylene glycol 6000 were prepared and evaluated. Seventy strains of homologous and heterologous beta- and non-beta-hemolytic streptococci were included in the evaluation. Homologous reactions were determined against extracts prepared by four extraction methods: hot hydrochloric acid (Lancefield) extraction, autoclave extraction, hot formamide extraction, and nitrous acid extraction. Enhancement of the precipitin reaction in the presence of polyethylene glycol 6000 permitted a fourfold dilution of all antisera for use in the capillary precipitin test. At this dilution, the potency of the antisera exceeded requirements established for these reagents.

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