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An improved procedure for identifying and quantitating protein phosphatases in mammalian tissues.

Authors
  • Cohen, P1
  • Klumpp, S
  • Schelling, D L
  • 1 Department of Biochemistry, University of Dundee, Scotland.
Type
Published Article
Journal
FEBS Letters
Publisher
Wiley (John Wiley & Sons)
Publication Date
Jul 03, 1989
Volume
250
Issue
2
Pages
596–600
Identifiers
PMID: 2546812
Source
Medline
License
Unknown

Abstract

The type 2A protein phosphatases in mammalian tissue extracts are inhibited completely and specifically by 1-2 nM okadaic acid. In contrast, type 1 protein phosphatases are hardly affected at these concentrations, complete inhibition requiring 1 microM okadaic acid. These observations have been exploited to develop an improved procedure for the identification and quantitation of type 1, type 2A and type 2C protein phosphatases in tissue extracts.

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