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An improved polychrome staining method for thick epoxy sections.

Authors
  • Jha, R K
Type
Published Article
Journal
Stain technology
Publication Date
May 01, 1976
Volume
51
Issue
3
Pages
159–162
Identifiers
PMID: 59424
Source
Medline
License
Unknown

Abstract

Improved polychrome staining of 1-1.5 mum epoxy sections is achieved with sequential applications of a single basic fuchsin-methylene blue mixture at two different pH values. The dye solution is applied for 2-3 min at 50-52 C first at pH 7.9, then at pH 6.7. In sections of mouse mammary tissue, epithelial cells are stained deep blue, connective tissue pink, and fat cells bright olive-green. This simple technique consistently yields uniform, vivid, contrasting colors that sharply delineate the elements of the complex glandular architecture of the mammary gland. Similar polychromatic effects are obtained in applications to other tissues, such as stomach, adrenal gland, mammary tumor and artery.

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