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Improved microradiographic contrast for bone stain-historadiography.

Authors
  • Simmons, D J
  • Bates, M
  • Teitelbaum, S L
Type
Published Article
Journal
Stain technology
Publication Date
May 01, 1976
Volume
51
Issue
3
Pages
153–157
Identifiers
PMID: 59423
Source
Medline
License
Unknown

Abstract

Microradiographs of 5-micron sections of methyl methacrylate embedded undemineralized bone show poor resolution, but prestaining with silver nitrate increases the radioopacity of the calcified tissues to soft x-rays without masking regional differences in microscopic mineralization. Identical results are achieved using aqueous (pH 5.8 and 7.5) or ammoniacal solutions (pH 7.9). Atomic absorption spectrometry detected no loss of calcium from the sections during staining. Osteoid in plastic-embedded bone is not rendered radiopaque by this technique even after prolonged staining times (5 min-2 hr).

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