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Improved methods for the preparation of high molecular weight DNA from large and small scale cultures of filamentous fungi.

Authors
  • Bainbridge, B W
  • Spreadbury, C L
  • Scalise, F G
  • Cohen, J
Type
Published Article
Journal
FEMS microbiology letters
Publication Date
Jan 01, 1990
Volume
54
Issue
1-3
Pages
113–117
Identifiers
PMID: 2323536
Source
Medline
License
Unknown

Abstract

Improved methods are described for the isolation of pure, high molecular weight DNA from small and large scale cultures of filamentous fungi. The methods depend on the extraction of DNA under conditions which prevent nuclease activity and contamination by carbohydrate. The small scale method depends on enzymatic digestion of the wall whereas the large scale method uses partial damage followed by autolysis. High yields of DNA are obtained by both methods and the DNA is suitable for restriction analysis. Southern Blotting, RFLP analysis, dot blotting and the production of gene libraries. The small scale method can be used for the simultaneous analysis of multiple cultures.

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