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An improved method for the rapid isolation of brain ribonucleic acid.

Authors
Type
Published Article
Journal
The Biochemical journal
Publication Date
Volume
183
Issue
1
Pages
181–184
Identifiers
PMID: 534484
Source
Medline
License
Unknown

Abstract

An improved one-step method for the extraction of RNA from rat brain is described. Fresh or frozen tissue is disrupted in the powerful protein denaturant guanidine thiocyanate, and RNA isolated by ultracentrifugation through CsCl. The procedure is advantageous in that it is relatively simple, is rapid and does not expose the sample to enzyme treatments or repeated organic extractions.

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