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An improved assay for interleukin 2 (lymphocyte growth factor) produced by mitogen-activated lymphocytes.

Authors
Type
Published Article
Journal
The Australian journal of experimental biology and medical science
Publication Date
Volume
58
Issue
6
Pages
533–544
Identifiers
PMID: 6791621
Source
Medline
License
Unknown

Abstract

The continued proliferation of activated T cells requires the presence of a lymphocyte growth factor in the culture medium. This study describes a rapid, highly reproducible assay to quantitatively measure levels of this lymphokine. The use of Concanavalin-A blast cells given this assay a high degree of flexibility and convenience. It is shown that the lymphokine measured is interleukin 2. The presence of an inhibitor in the supernatant of mitogen activated lymphocytes and the species specificity of the factor are demonstrated.

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