Affordable Access

Implication of acid cholesteryl ester hydrolase in the regulation of steroid biosynthesis in rat Leydig cells.

Authors
  • Löffler, B M
  • Kunze, H
Type
Published Article
Journal
Biochemistry international
Publication Date
Dec 01, 1992
Volume
28
Issue
6
Pages
965–974
Identifiers
PMID: 1337827
Source
Medline
License
Unknown

Abstract

Cholesteryl ester hydrolase (CEH) activities with different pH optima, i.e. at about pH 4.0 and pH 7.0, were found in Leydig cells isolated from rat testis. Highly purified intact Leydig cells responded to human chorionic gonadotropin treatment with increased intracellular cAMP levels, increased testosterone release, and a 1.5-fold stimulation of the acid CEH activity. Treatment of non-stimulated homogenised cells with cAMP-dependent proteinkinase, cAMP and ATP lead to a 3.0-fold stimulation of the acid CEH activity. These findings suggest that interconvertible CEH with an acidic pH optimum--presumably of lysosomal location--contributes to the regulation of steroidogenesis in Leydig cells.

Report this publication

Statistics

Seen <100 times