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Impairing the radioresistance of cancer cells by hydrogenated nanodiamonds.

Authors
  • Grall, Romain
  • Girard, Hugues
  • Saad, Lina
  • Petit, Tristan
  • Gesset, Céline
  • Combis-Schlumberger, Mathilde
  • Paget, Vincent
  • Delic, Jozo
  • Arnault, Jean-Charles
  • Chevillard, Sylvie
Type
Published Article
Journal
Circulation
Volume
61
Pages
290–298
Identifiers
DOI: 10.1016/j.biomaterials.2015.05.034
PMID: 26010122
Source
USPC - SET - SVS
Keywords
License
Unknown

Abstract

Hydrogenated nanodiamonds (H-NDs) exhibit a negative electron affinity that confers a high reactivity with oxygen species and a positive charge in aqueous solutions. It allows electron emission from H-NDs following irradiation by photons and in consequence may enhance the effects of radiation on cancer cells. By using three human radioresistant cancer cell lines, we showed a potentialization of cytotoxicity after a co-exposure to H-NDs and irradiation; an event occurring through the induction of DNA damage and reactive oxygen species. This occurred together with a decrease in cell impedance, the activation of G1/S, an unlocking of G2 cell cycle check-points and early low cell death rate. At later stage of exposure, persistent increases in heterochromatinization, large γ-H2AX foci and β-galactosidase activity were detected providing evidence of cells' entrance into senescence. Similar potentialization was observed with neocarzinostatin (NCS), a radiomimetic drug. This original finding underlines a wide clinical potential of H-NDs to intensify radiation effects on radio-resistant cancer cells.

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