Affordable Access

deepdyve-link
Publisher Website

Impaired Exocrine Pancreatic Function Associates With Changes in Intestinal Microbiota Composition and Diversity.

Authors
  • Frost, Fabian1
  • Kacprowski, Tim2
  • Rühlemann, Malte3
  • Bülow, Robin4
  • Kühn, Jens-Peter5
  • Franke, Andre3
  • Heinsen, Femke-Anouska3
  • Pietzner, Maik6
  • Nauck, Matthias7
  • Völker, Uwe8
  • Völzke, Henry9
  • Aghdassi, Ali A1
  • Sendler, Matthias1
  • Mayerle, Julia10
  • Weiss, Frank U1
  • Homuth, Georg8
  • Lerch, Markus M11
  • 1 Department of Medicine A, University Medicine Greifswald, Greifswald, Germany. , (Germany)
  • 2 Department of Functional Genomics, Interfaculty Institute for Genetics and Functional Genomics, University Medicine Greifswald, Greifswald, Germany; Chair of Experimental Bioinformatics, TUM School of Life Sciences Weihenstephan, Technical University of Munich, Freising, Germany; DZHK (German Centre for Cardiovascular Research), Partner Site Greifswald, University Medicine Greifswald, Greifswald, Germany. , (Germany)
  • 3 Institute of Clinical Molecular Biology, Christian Albrechts University of Kiel, Kiel, Germany. , (Germany)
  • 4 Department of Diagnostic Radiology and Neuroradiology, University Medicine Greifswald, Greifswald, Germany. , (Germany)
  • 5 Department of Diagnostic Radiology and Neuroradiology, University Medicine Greifswald, Greifswald, Germany; Institute of Radiology, University Medicine, Carl-Gustav-Carus University, Dresden, Germany. , (Germany)
  • 6 Institute of Clinical Chemistry and Laboratory Medicine, University Medicine Greifswald, Greifswald, Germany. , (Germany)
  • 7 DZHK (German Centre for Cardiovascular Research), Partner Site Greifswald, University Medicine Greifswald, Greifswald, Germany; Institute of Clinical Chemistry and Laboratory Medicine, University Medicine Greifswald, Greifswald, Germany. , (Germany)
  • 8 Department of Functional Genomics, Interfaculty Institute for Genetics and Functional Genomics, University Medicine Greifswald, Greifswald, Germany. , (Germany)
  • 9 Institute for Community Medicine, University Medicine Greifswald, Greifswald, Germany. , (Germany)
  • 10 Department of Medicine A, University Medicine Greifswald, Greifswald, Germany; Department of Medicine II, University Hospital, LMU Munich, Germany. , (Germany)
  • 11 Department of Medicine A, University Medicine Greifswald, Greifswald, Germany. Electronic address: [email protected] , (Germany)
Type
Published Article
Journal
Gastroenterology
Publication Date
Nov 02, 2018
Identifiers
DOI: 10.1053/j.gastro.2018.10.047
PMID: 30391469
Source
Medline
Keywords
Language
English
License
Unknown

Abstract

Changes in intestinal microbiome composition are associated with inflammatory, metabolic, and malignant disorders. We studied how exocrine pancreatic function affects intestinal microbiota. We performed 16S ribosomal RNA gene sequencing analysis of stool samples from 1795 volunteers from the population-based Study of Health in Pomerania who had no history of pancreatic disease. We also measured fecal pancreatic elastase by enzyme-linked immunosorbent assay and performed quantitative imaging of secretin-stimulated pancreatic fluid secretion. Associations of exocrine pancreatic function with microbial diversity or individual genera were calculated by permutational analysis of variance or linear regression, respectively. Differences in pancreatic elastase levels associated with significantly (P < .0001) greater changes in microbiota diversity than with participant age, body mass index, sex, smoking, alcohol consumption, or dietary factors. Significant changes in the abundance of 30 taxa, such as an increase in Prevotella (q < 0.0001) and a decrease of Bacteroides (q < 0.0001), indicated a shift from a type-1 to a type-2 enterotype. Changes in pancreatic fluid secretion alone were also associated with changes in microbial diversity (P = .0002), although to a lesser degree. In an analysis of fecal samples from 1795 volunteers, pancreatic acinar cell, rather than duct cell, function is presently the single most significant host factor to be associated with changes in intestinal microbiota composition. Copyright © 2019 AGA Institute. Published by Elsevier Inc. All rights reserved.

Report this publication

Statistics

Seen <100 times