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Impact of Transcriptional Regulation by Crp, FruR, FlhD, and TyrR on L-tryptophan Biosynthesis in Escherichia coli

Authors
  • Liu, L.1
  • Bilal, M.1
  • Luo, H.1
  • Iqbal, H. M. N.2
  • 1 School of Life Science and Food Engineering, Huaiyin Institute of Technology, Huaian, 223003, China , Huaian (China)
  • 2 Tecnologico de Monterrey, School of Engineering and Sciences, Monterrey, 64849, Mexico , Monterrey (Mexico)
Type
Published Article
Journal
Applied Biochemistry and Microbiology
Publisher
Pleiades Publishing
Publication Date
May 01, 2021
Volume
57
Issue
3
Pages
319–326
Identifiers
DOI: 10.1134/S0003683821030091
Source
Springer Nature
Keywords
License
Yellow

Abstract

AbstractTranscriptional regulation plays a pivotal role in metabolic flow, which can, in turn, affect L-Trp biosynthesis in Escherichia coli. The application of global transcriptional regulation by Crp, FruR, FlhD, and TyrR for L-Trp biosynthesis during shake-flask fermentation of E. coli FB-04/pSV03, an L-Trp-producing strain, was elucidated. Inactivation of TyrR was phenotypically silent, whereas, absence of the Crp caused a noticeable reduction in L-Trp yield and a pronounced slow-growth phenotype. Lacking FlhD led to a decrease in the acetate level; however, the L-Trp yield also decreased. When FruR was inactivated, the L-Trp biosynthesis and the biotransformation rate were improved by 59.5 and 52.4%, respectively. However, using the same strain with deleted fruR-gene, for fed-batch cultivation in a 3-L reactor leads to a significant decrease in L-Trp biosynthesis and the conversion rate.

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