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The impact of StuI digestion in situ on FISH to human chromosomes with satellite DNA probes.

Authors
  • Nieddu, M
  • Pichiri, G
  • Melis, V
  • Mezzanotte, R
Type
Published Article
Journal
Heredity
Publisher
Springer Nature
Publication Date
Apr 01, 2003
Volume
90
Issue
4
Pages
298–301
Identifiers
PMID: 12692582
Source
Medline
License
Unknown

Abstract

Human metaphase chromosomes were digested with StuI and subsequently hybridized in situ using chromosome 9 alphoid DNA and classical satellite III DNA as probes. The data obtained suggest that it is not possible to establish a general rule regarding the cytological effects induced by restriction enzymes in particular chromosome regions and that a number of factors, such as DNA sequences, DNA-protein interaction and enzyme structure, play a role in determining such effects.

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