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Transgenic RXLR Effector PITG_15718.2 Suppresses Immunity and Reduces Vegetative Growth in Potato.

Authors
  • Wang, Jiao1, 2
  • Gao, Cungang3, 4
  • Li, Long5
  • Cao, Weilin6, 7
  • Dong, Ran8
  • Ding, Xinhua9, 10
  • Zhu, Changxiang11, 12
  • Chu, Zhaohui13, 14
  • 1 State Key Laboratory of Crop Biology, Shandong Agricultural University, Tai'an 271018, China. [email protected] , (China)
  • 2 Shandong Provincial Key Laboratory of Vegetable Disease and Insect Pests, College of Plant Protection, Shandong Agricultural University, Tai'an 271018, China. [email protected] , (China)
  • 3 State Key Laboratory of Crop Biology, Shandong Agricultural University, Tai'an 271018, China. [email protected] , (China)
  • 4 College of Agronomy, Shandong Agricultural University, Tai'an 271018, China. [email protected] , (China)
  • 5 College of Agronomy, Shandong Agricultural University, Tai'an 271018, China. [email protected] , (China)
  • 6 State Key Laboratory of Crop Biology, Shandong Agricultural University, Tai'an 271018, China. [email protected] , (China)
  • 7 College of Life Science, Shandong Agricultural University, Tai'an, 271018, China. [email protected] , (China)
  • 8 Shandong Provincial Key Laboratory of Vegetable Disease and Insect Pests, College of Plant Protection, Shandong Agricultural University, Tai'an 271018, China. [email protected] , (China)
  • 9 State Key Laboratory of Crop Biology, Shandong Agricultural University, Tai'an 271018, China. [email protected] , (China)
  • 10 Shandong Provincial Key Laboratory of Vegetable Disease and Insect Pests, College of Plant Protection, Shandong Agricultural University, Tai'an 271018, China. [email protected] , (China)
  • 11 State Key Laboratory of Crop Biology, Shandong Agricultural University, Tai'an 271018, China. [email protected] , (China)
  • 12 College of Life Science, Shandong Agricultural University, Tai'an, 271018, China. [email protected] , (China)
  • 13 State Key Laboratory of Crop Biology, Shandong Agricultural University, Tai'an 271018, China. [email protected] , (China)
  • 14 College of Agronomy, Shandong Agricultural University, Tai'an 271018, China. [email protected] , (China)
Type
Published Article
Journal
International Journal of Molecular Sciences
Publisher
MDPI AG
Publication Date
Jun 21, 2019
Volume
20
Issue
12
Identifiers
DOI: 10.3390/ijms20123031
PMID: 31234322
Source
Medline
Keywords
Language
English
License
Unknown

Abstract

Phytophthora infestans causes the severe late blight disease of potato. During its infection process, P. infestans delivers hundreds of RXLR (Arg-x-Leu-Arg, x behalf of any one amino acid) effectors to manipulate processes in its hosts, creating a suitable environment for invasion and proliferation. Several effectors interact with host proteins to suppress host immunity and inhibit plant growth. However, little is known about how P. infestans regulates the host transcriptome. Here, we identified an RXLR effector, PITG_15718.2, which is upregulated and maintains a high expression level throughout the infection. Stable transgenic potato (Solanum tuberosum) lines expressing PITG_15718.2 show enhanced leaf colonization by P. infestans and reduced vegetative growth. We further investigated the transcriptional changes between three PITG_15718.2 transgenic lines and the wild type Désirée by using RNA sequencing (RNA-Seq). Compared with Désirée, 190 differentially expressed genes (DEGs) were identified, including 158 upregulated genes and 32 downregulated genes in PITG_15718.2 transgenic lines. Eight upregulated and nine downregulated DEGs were validated by real-time RT-PCR, which showed a high correlation with the expression level identified by RNA-Seq. These DEGs will help to explore the mechanism of PITG_15718.2-mediated immunity and growth inhibition in the future.

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