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Impact of nutrition and rotavirus infection on the infant gut microbiota in a humanized pig model

Authors
  • Kumar, Anand1, 2
  • Vlasova, Anastasia N.1
  • Deblais, Loic1
  • Huang, Huang-Chi1
  • Wijeratne, Asela3
  • Kandasamy, Sukumar1
  • Fischer, David D.1
  • Langel, Stephanie N.1
  • Paim, Francine Chimelo1
  • Alhamo, Moyasar A.1
  • Shao, Lulu1, 4
  • Saif, Linda J.1
  • Rajashekara, Gireesh1
  • 1 The Ohio State University, Food Animal Research Program, The Ohio Agricultural Research and Development Center,Veterinary Preventive Medicine Department, 1680 Madison Avenue, Wooster, OH, 44691, USA , Wooster (United States)
  • 2 Present address: Group B-10: Biosecurity and Public Health, Bioscience Division, Los Alamos National Laboratory, Los Alamos, NM, USA , Los Alamos (United States)
  • 3 The Ohio State University, The Molecular and Cellular Imaging Center, Wooster, OH, USA , Wooster (United States)
  • 4 University of Pittsburgh, Present address: Hillman Cancer Center, 4200 Fifth Ave, Pittsburgh, PA, 15260, USA , Pittsburgh (United States)
Type
Published Article
Journal
BMC Gastroenterology
Publisher
Springer (Biomed Central Ltd.)
Publication Date
Jun 22, 2018
Volume
18
Issue
1
Identifiers
DOI: 10.1186/s12876-018-0810-2
Source
Springer Nature
Keywords
License
Green

Abstract

BackgroundHuman rotavirus (HRV) is a major cause of viral gastroenteritis in infants; particularly in developing countries where malnutrition is prevalent. Malnutrition perturbs the infant gut microbiota leading to sub-optimal functioning of the immune system and further predisposing infants to enteric infections. Therefore, we hypothesized that malnutrition exacerbates rotavirus disease severity in infants.MethodsIn the present study, we used a neonatal germ free (GF) piglets transplanted with a two-month-old human infant’s fecal microbiota (HIFM) on protein deficient and sufficient diets. We report the effects of malnourishment on the HRV infection and the HIFM pig microbiota in feces, intestinal and systemic tissues, using MiSeq 16S gene sequencing (V4-V5 region).ResultsMicrobiota analysis indicated that the HIFM transplantation resulted in a microbial composition in pigs similar to that of the original infant feces. This model was then used to understand the interconnections between microbiota diversity, diet, and HRV infection. Post HRV infection, HIFM pigs on the deficient diet had lower body weights, developed more severe diarrhea and increased virus shedding compared to HIFM pigs on sufficient diet. However, HRV induced diarrhea and shedding was more pronounced in non-colonized GF pigs compared to HIFM pigs on either sufficient or deficient diet, suggesting that the microbiota alone moderated HRV infection. HRV infected pigs on sufficient diet showed increased microbiota diversity in intestinal tissues; whereas, greater diversity was observed in systemic tissues of HRV infected pigs fed with deficient diet.ConclusionsThese results suggest that proper nourishment improves the microbiota quality in the intestines, alleviates HRV disease and lower probability of systemic translocation of potential opportunistic pathogens/pathobionts. In conclusion, our findings further support the role for microbiota and proper nutrition in limiting enteric diseases.

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