AbstractThe effect of the endocrine disruptor nonylphenol on the biological activity of loamy soddy-podzolic soil (Eutric Albic Retisol (Abruptic, Loamic, Aric, Ochric)) was studied in model experiments. The upper horizons of loamy soddy-podzolic soil sampled in Leningrad oblast were the object of this study. The number of soil microorganisms, the activity of soil enzymes, and soil respiration were determined with the methods commonly used in soil microbiology and biochemistry. The content of nonylphenol in soil samples was determined using high-performance liquid chromatography. Soil contamination with nonylphenol was shown to cause time- and dose-dependent changes in the activity of the pool of nitrogen, carbohydrates, and redox enzymes and increases the basal respiration. The results obtained are an important stage necessary for risk assessment of soil contamination with alkylphenols, and can be used in the development of a remediation methodology for soils contaminated with endocrine disruptors.