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The Impact of Nicotine and Cigarette Smoke Condensate on Metabolic Activity and Biofilm Formation of Candida albicans on Acrylic Denture Material.

Authors
  • Alzayer, Yasmin Mohammed1
  • Gomez, Grace F2
  • Eckert, George J3
  • Levon, John A1
  • Gregory, Richard L2
  • 1 Department of Prosthodontics, Indiana University School of Dentistry, Indianapolis, IN. , (India)
  • 2 Department of Biomedical and Applied Sciences, Indiana University School of Dentistry, Indianapolis, IN. , (India)
  • 3 Department of Biostatistics, Indiana University School of Medicine, Indianapolis, IN. , (India)
Type
Published Article
Journal
Journal of prosthodontics : official journal of the American College of Prosthodontists
Publication Date
Feb 01, 2020
Volume
29
Issue
2
Pages
173–178
Identifiers
DOI: 10.1111/jopr.12945
PMID: 30028051
Source
Medline
Keywords
Language
English
License
Unknown

Abstract

Smokers have increased denture stomatitis caused primarily by Candida albicans. The primary aim of this study was to demonstrate the impact of a wide range of nicotine and cigarette smoke condensate (CSC) concentrations on biofilm formation and metabolic activity of C. albicans on acrylic denture material. C. albicans (ATCC strain 10231) was used. Standardized denture acrylic (PMMA) specimens (total of 135 specimens) were incubated with C. albicans and exposed to nicotine and CSC at different concentrations (0, 0.25, 0.5, 1, 2, 4, 8, 16, and 32 mg/ml) and (0, 0.25, 0.5, 1, 2, and 4 mg/ml), respectively. For each experiment, 3 samples per nicotine and CSC concentration and a total of 45 specimens (27 specimens for the nicotine and 18 specimens for the CSC-treated samples) were used and were selected randomly for each group. The control group consisted of 0 mg/ml of nicotine or CSC. The viability of C. albicans was measured using spiral plating on blood agar plates. The effect of nicotine and CSC concentrations on planktonic cells was were measured using a microplate reader. Metabolic activity of 24-hour-old established C. albicans biofilm exposed to nicotine and CSC for 24 hours in microtiter plates was determined using a 2,3-bis (2-methoxy-4-nitro-5-sulfophenyl)-2H-tetrazolium-carboxanilide (XTT) reduction assay. The viability of C. albicans increased concomitant with increasing concentrations of CSC and nicotine, particularly at 0.5 and 2 mg/ml, respectively. Concentrations of CSC and nicotine above this resulted in an inhibitory effect on C. albicans viability. CSC and nicotine at 4 and 16 mg/ml, respectively, increased C. albicans biofilm metabolic activity. Nicotine and CSC up to certain concentrations caused increases in biofilm formation, metabolic activity, viability, and planktonic cell absorbance of C. albicans. This in vitro study demonstrates the effectiveness of tobacco on promoting the growth of C. albicans and suggests their potential contributing factor in C. albicans biofilm related infections in smokers. © 2018 by the American College of Prosthodontists.

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