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Impact of minocycline ointment for periodontal treatment of oral bacteria.

Authors
  • Nakao, Ryoma
  • Takigawa, Satoko
  • Sugano, Naoyuki
  • Koshi, Ryosuke
  • Ito, Koichi
  • Watanabe, Haruo
  • Senpuku, Hidenobu
Type
Published Article
Journal
Japanese Journal of Infectious Diseases
Publisher
"Editorial Committee of Japanese Journal of Infectious Diseases, National Institute of Infectious Diseases"
Publication Date
Jan 01, 2011
Volume
64
Issue
2
Pages
156–160
Identifiers
PMID: 21519133
Source
Medline
License
Unknown

Abstract

Topical tetracyclines, such as minocycline ointment, are frequently used for the treatment of periodontal infection. We investigated the influence of minocycline ointment use on oral bacteria, using supragingival plaque samples from adults who had not taken any antibiotics for 6 months. Initially we investigated the effect of topical minocycline administration on the emergence of tetracycline-resistant oral bacteria in four healthy adults. The isolation frequency of tetracycline-resistant oral bacteria to total viable bacteria increased substantially on day 6 after treatment, although it returned to baseline on day 25. Subsequently we investigated the isolation frequency of tetracycline-resistant oral streptococci (TOS) as a representative oral bacterium, using samples from 41 subjects with periodontal diseases. The percentage of TOS (of the total oral streptococci) increased significantly (from 11.9±15.6% to 34.2±24.0%) after minocycline treatment. Various TOS species were identified; S. mitis, S. salivarius, S. sanguinis, and S. oralis were frequently isolated. PCR and Southern blotting allowed us to identify tetM on the Tn916-like elements as the gene responsible for tetracycline-resistance. These findings suggest that the potential risk of the spread of similar genetic elements through bacteria in the oral cavity should be considered.

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