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Impact of cardiac resynchronization therapy on circulating IL-17 producing cells in patients with advanced heart failure

Authors
  • Martins, Sílvia1, 2
  • Carvalheiro, Tiago3
  • Laranjeira, Paula2, 4
  • Martinho, António3
  • Elvas, Luís5, 6
  • Gonçalves, Lino5, 6
  • Tomaz, Cândida1, 7
  • António, Natália5, 6
  • Paiva, Artur2, 4, 8, 9
  • 1 Universidade da Beira Interior, CICS-UBI – Centro de Investigação em Ciências da Saúde, Covilhã, Portugal , Covilhã (Portugal)
  • 2 Faculdade de Medicina, Coimbra Institute for Clinical and Biomedical Research, Coimbra, Portugal , Coimbra (Portugal)
  • 3 Instituto Português do Sangue e da Transplantação, Centro do Sangue e da Transplantação de Coimbra, Coimbra, Portugal , Coimbra (Portugal)
  • 4 Centro Hospitalar e Universitário de Coimbra, Flow Cytometry Unit, Department of Clinical Pathology, Coimbra, Portugal , Coimbra (Portugal)
  • 5 Coimbra Hospital and Universitary Centre, Cardiology Department, Coimbra, Portugal , Coimbra (Portugal)
  • 6 Coimbra University, Faculty of Medicine, Coimbra, Portugal , Coimbra (Portugal)
  • 7 Universidade da Beira Interior, Departamento de Química, Covilhã, Portugal , Covilhã (Portugal)
  • 8 ESTESC - Coimbra Health School, Ciências Biomédicas Laboratoriais, Instituto Politécnico de Coimbra, Coimbra, Portugal , Coimbra (Portugal)
  • 9 Centro Hospitalar e Universitário de Coimbra, Unidade de Gestão Operacional de Citometria, Serviço de Patologia Clínica, Ed. S. Jerónimo, Praceta Mota Pinto, Coimbra, 3001-301, Portugal , Coimbra (Portugal)
Type
Published Article
Journal
Journal of Interventional Cardiac Electrophysiology
Publisher
Springer-Verlag
Publication Date
Nov 27, 2018
Volume
54
Issue
3
Pages
257–265
Identifiers
DOI: 10.1007/s10840-018-0491-3
Source
Springer Nature
Keywords
License
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Abstract

PurposeIL-17-producing T cells have been implicated in the inflammatory milieu of chronic heart failure (CHF), which implies a dismal prognosis in affected patients. The aim of this study was to evaluate the impact of cardiac resynchronization therapy (CRT) on the frequency and functional activity of Th17 and Tc17 cells, as well as, on IL-17 mRNA expression in patients with CHF.MethodsTwenty-eight patients with CHF, analyzed before CRT (T0) and 6 months later (T6), and 15 healthy controls (HC) were enrolled in this study. Circulating Th17 and Tc17 cells were evaluated by flow cytometry. The quantification of IL-17A mRNA expression was performed by real-time PCR.ResultsCirculating Tc17 cells tended to be higher in CHF patients submitted to CRT than in HC (0.92% (0.24–3.32) versus 0.60% (0.09–3.68), although not reaching statistical significance. The frequency of Tc17 cells in CHF patients significantly decreases after CRT reaching levels similar to those of HC (0.92% (0.24–3.32) at T0 versus 0.56% (0.21–4.20) at T6, P < 0.05), mainly due to responders to CRT. Additionally, the expression of IL-17 mRNA was detected in a few number of responder patients at T0 (27%) and only detected in one responder at T6 (7%). Conversely, in non-responders, the proportion of patients exhibiting IL-17 mRNA expression increases from baseline (17%) to T6 (42%). No significant differences were observed in Th17 cells between HC, CHF patients in T0 and patients in T6.ConclusionThe inflammatory response mediated by circulating IL-17 producing cells seems to be suppressed by CRT, particularly in responders.

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