Biosurfactant is a structurally diverse group of surface-active molecule, synthesized by microorganisms. Kocuria rosea and Pseudomonas aeruginosa strains isolated from pesticide contaminated soil, which produces biosurfactant were studied. Curd whey was used as a cheap source of growth medium for biosurfactant production. There was formation of stable emulsions of biosurfactant containing broth with vegetable oil and kerosene. These strains produced a clear zone in oil spreading test, which is an indicative of the good biosurfactant activity. Both the strains produced extra cellular biosurfactant in the culture media and showed good foam stability in the culture medium. Biosurfactant was efficiently extracted from the culture broth by acetone-HCl precipitation. The biosurfactants from the two species, namely Kocuria rosea and Pseudomonas aeruginosa were found to have no effects on germinating seedlings of Glycine max, Pisum sativum and Spinacia oleracea, when treated with 25%, 50%, 75% and 100% with the combination of curd whey in the making of 100ml volume. Curd whey as a control was taken with no surfactant. Our study suggested an efficient use in surfactant aided bioremediation in agricultural land.