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Impact of an anti-Salmonella. Typhimurium Bacteriophage on intestinal microbiota and immunity status of laying hens.

Authors
  • Lee, MiJin1
  • Hosseindoust, Abdolreza2
  • Oh, SeungMin3
  • Ko, HanSeo2
  • Cho, EunSeok1
  • Sa, SooJin1
  • Kim, YoungIn4
  • Choi, JungWoo2
  • Kim, JinSoo2
  • 1 Poultry Science Division, Livestock Research Development, National Institute of Animal Science, RDA, Cheonan, Korea. , (North Korea)
  • 2 College of Animal Life Sciences, Kangwon National University, Chuncheon, Korea. , (North Korea)
  • 3 Gyeongsangbuk-do Livestock Research Institute, Yeongju, Korea. , (North Korea)
  • 4 CTC Bio, Inc., Seoul, Korea. , (North Korea)
Type
Published Article
Journal
Journal of animal physiology and animal nutrition
Publication Date
Sep 01, 2021
Volume
105
Issue
5
Pages
952–959
Identifiers
DOI: 10.1111/jpn.13424
PMID: 32772452
Source
Medline
Keywords
Language
English
License
Unknown

Abstract

Typhoid is a distinct gastrointestinal disease that largely affects the public by consumption of inadequately or partially cooked eggs from contaminated laying hen farms. This has led the research on laying hens to focus on controlling the contamination by an effective anti-Salmonella spp. agent in the intestine. The treatments included, control, without challenge; PC, Salmonella typhimurium challenged (STC); BP5, 5 ppm bacteriophage/kg + STC; BP10, 10 ppm bacteriophage/kg + STC, on Salmonella shedding, body organs inflammatory reactions, and expression of toll-like receptor (TLR), pro-inflammatory cytokines, and heat shock protein (HSP) in the jejunum, liver,and thigh muscle in the STC laying hens. The RT-PCR method was used to enumerate the number of Salmonella typhimurium in the organs. The birds in the STC groups exhibited the increased population of Salmonella spp. in the excreta (p < .01). In the STC groups, the BP5 and BP10 laying hens exhibited a lower (p < .01) population of Salmonella spp. in the excreta at d 7 after STC. Supplementation of bacteriophage significantly decreased (p < .01) the colonization of S. Typhimurium in the spleen, oviduct, caecum and excreta. Among the STC treatments, the BP10 laying hens showed lower (p < .01) mRNA expression of interferon-γ (IFNγ) and TLR-4 in the jejunum compared with the PC treatment. After the STC, dietary supplementation with BP5 or BP10 decreased (p < .01) the mRNA expressions of IFNγ, HSP-27 and tumour necrosis factor-α in the liver compared with the PC treatment. These results suggest that bacteriophage can be used as an effective agent to decrease S. Typhimurium contamination in laying hens and possibly lower S. Typhimurium transfer to foods. © 2020 Blackwell Verlag GmbH.

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