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Immunoquantitation of cytochrome b5 and methylcholanthrene-induced cytochromes P-450.

Authors
  • Shires, T K
  • Krieter, P A
  • Shawver, L K
  • Seidel, S L
Type
Published Article
Journal
Federation proceedings
Publication Date
Jun 01, 1987
Volume
46
Issue
8
Pages
2567–2574
Identifiers
PMID: 2439385
Source
Medline
License
Unknown

Abstract

The enzyme-linked immunosorbent assay (ELISA) has been investigated for its ability to quantitate hydrophobic proteins like cytochromes b5 and P-450 at the subnanogram level. Issues encountered that have broad significance not only for ELISA, but for other qualitative and quantitative immunoassays as well, include the effects of detergent, the discriminatory capacity of ELISA, and the method for determining an assay's selectivity.

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