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Immunological characterization of honey proteins and identification of MRJP 1 as an IgE-binding protein.

Authors
  • Hayashi, Takeshi
  • Takamatsu, Nobue
  • Nakashima, Takashi
  • Arita, Takashi
Type
Published Article
Journal
Bioscience Biotechnology and Biochemistry
Publisher
Informa UK (Taylor & Francis)
Publication Date
Jan 01, 2011
Volume
75
Issue
3
Pages
556–560
Identifiers
PMID: 21389615
Source
Medline
License
Unknown

Abstract

We encountered a fourth case of honey allergy in Japan. We characterized and identified the IgE-binding proteins in honey using the serum of a honey-allergenic patient. Immunoblot analysis revealed that IgE in the patient serum specifically bound to four proteins in each honey sample. At least three of these IgE-binding proteins were N-linked glycoproteins. To identify the 60-kDa IgE-binding protein in dandelion honey, the N-terminal sequences of the fragmented protein were analyzed, revealing the protein to be major royal jelly protein 1 (MRJP 1). Three IgE-binding proteins removed of N-linked oligosaccharide showed a large reduction in IgE-binding activity as compared with the intact protein. This suggests that the carbohydrates in the IgE-binding proteins are a major epitope for patient IgE.

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