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Immunolocalization of cholesterol side chain cleavage enzyme (P450scc) in Mytilus galloprovincialis and its induction by nutritional levels.

Authors
  • Martínez, Ana Alonso
  • Muñoz, Yolanda Ruiz
  • Serrano, Fuencisla San Juan
  • García, Pilar Molist
Type
Published Article
Journal
Journal of comparative physiology. B, Biochemical, systemic, and environmental physiology
Publication Date
Jul 01, 2008
Volume
178
Issue
5
Pages
647–654
Identifiers
DOI: 10.1007/s00360-008-0256-x
PMID: 18340451
Source
Medline
License
Unknown

Abstract

We have examined the localization of P450scc in different tissues of the mollusc Mytilus galloprovincialis along a gonadal cycle by using a polyclonal antibody against rat cholesterol side-chain cleavage enzyme (cytochrome P450scc). Immunoreactivity specific for P450scc was found only in the cytoplasm of basophilic cells from digestive gland; gonad, gills and kidney appeared to be devoid of P450scc immunoreactivity. SDS-gel electrophoresis and western blot analysis of different subcellular fractions revealed that this protein is mainly located in microsomes, has a molecular weight of 51.4 kDa and is recognized by the antibody against rat P450scc. These results suggest that the digestive gland of M. galloprovincialis could be considered a steroidogenic tissue where the first step in biosynthesis of steroid hormones occurs. Moreover, seasonal slot blot analysis of post-mitochondrial fractions showed a P450scc induction by available nutrients and phytoplankton blooms.

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