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Immunohistochemical and ultrastructural studies of the effects of prednisolone on transformation of fibroblast to regenerated mesothelial cells

Authors
  • Amari, Masao1
  • Taguchi, Katsuji2
  • Iwahara, Minoru2
  • Naoe, Shiro3
  • Takahasi, Key2
  • 1 Toho University School of Medicine, Division of Electron Microscopy, Ohashi Hospital, 2-17-6 Ohashi, Meguro-ku, Tokyo, 153-8515, Japan , Tokyo
  • 2 Toho University School of Medicine, Department of Pathology, Ohashi Hospital, Tokyo, Japan , Tokyo
  • 3 Toin University of Yokohama, Department of Biomedical Engineering, Faculty of Engineering, Japan
Type
Published Article
Journal
Medical Electron Microscopy
Publisher
Springer-Verlag
Publication Date
Dec 01, 2004
Volume
37
Issue
4
Pages
242–251
Identifiers
DOI: 10.1007/s00795-004-0266-z
Source
Springer Nature
Keywords
License
Yellow

Abstract

We have proposed in the past that chest wall fibroblasts are transformed to regenerated mesothelial cells. This study was conducted to investigate the effects of prednisolone on the differentiation and migration of fibroblasts in their transformation to mesothelial cells. Rat fibroblasts harvested from intercostal thoracic wall specimens were cultured in culture medium until cell spheroids were formed. An experimental cell spheroid group to whose culture medium prednisolone had been added and a control spheroid group with no addition of prednisolone were then subjected to immunohistochemical and ultrastructural studies of the changes in the fibroblasts with the passage of time. On days 1 and 2 of culture, the fibroblasts in each group were cytokeratin negative. However, on day 3 the control group became cytokeratin positive, and ultrastructural observations revealed formation of macula adherens and microvilli. In contrast, the experimental group fibroblasts remained cytokeratin negative even on day 3, but became cytokeratin positive on day 5 of culture. Macula adherens and microvilli also manifested on day 5. Prednisolone inhibited the differentiation and migration of fibroblasts, but it was surmised that fibroblasts that have resisted from the effects of prednisolone finally differentiate into mesothelial cells which have formed macula adherens.

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