Affordable Access

Immunofluorescence localization of nuclear retinoid receptors in psoriasis versus normal human skin.

Authors
  • Karlsson, Teresa
  • Rollman, Ola
  • Vahlquist, Anders
  • Törmä, Hans
Type
Published Article
Journal
Acta Dermato Venereologica
Publisher
Acta Dermato-Venereologica
Publication Date
Jan 01, 2004
Volume
84
Issue
5
Pages
363–369
Identifiers
PMID: 15370702
Source
Medline
License
Unknown

Abstract

Psoriasis responds favourably to treatment with retinoids but the cellular pathways mediating these effects are poorly understood. Retinoids regulate keratinocyte proliferation and maturation via binding to nuclear retinoic acid receptors (mainly RARalpha and RARgamma) which form heterodimers with the 9-cis-RA receptor, RXRalpha. We have previously shown that mRNA expression of RARalpha and RXRalpha is down-regulated in psoriatic lesions as compared with non-lesional human skin. In the present study, we investigated the protein expression of RARalpha, RARgamma and RXRalpha in normal and psoriatic skin using indirect immunofluorescence analysis. Epidermal keratinocytes of normal and non-lesional psoriatic skin displayed similar nuclear localization of all three receptors; RARalpha was detected with decreasing intensity from basal to suprabasal layers, RARgamma showed the opposite trend, whereas RXRalpha was evenly expressed throughout the epidermis. In lesional psoriatic skin, however, all three receptor proteins showed a much higher staining intensity in the lower half of the epidermis; in particular, RARalpha immunoreactivity was low or even absent in the upper layers of epidermis. The results support the idea that psoriasis is associated with abnormal retinoid signalling in lesional epidermis.

Report this publication

Statistics

Seen <100 times