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Immunoelectron microscopy analysis of HCMV gpUL73 (gN) localization.

Authors
  • 1
  • 1 Department of Clinical and Experimental Medicine, Division of Microbiology and Virology, University of Bologna, St. Orsola General Hospital, Italy. , (Italy)
Type
Published Article
Journal
Archives of virology
Publication Date
Volume
147
Issue
6
Pages
1247–1256
Identifiers
PMID: 12111433
Source
Medline
License
Unknown

Abstract

Human Cytomegalovirus (HCMV) UL73 encodes for a polymorphic structural glycoprotein, gpUL73(gN), conserved among herpesviruses. This study analyzed the intracellular and intraviral localization of gpUL73 by immunoelectron-microscopy comparing the reactivity of two different antibodies. We found that gN is an envelope component of the mature viral particle with at least a portion exposed at the virus surface and another at the internal side of the envelope. Furthermore, gpUL73 is also present in the matrix of dense bodies and "black holes". These results, as well as immunoblotting analysis, suggest that the two antibodies recognize different forms, fully processed or unprocessed, of gpUL73-gN.

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