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Immunoelectron microscopic study for polyamines.

Authors
  • Fujiwara, K
  • Bai, G
  • Kitagawa, T
  • Tsuru, D
Type
Published Article
Journal
The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society
Publication Date
Nov 01, 1998
Volume
46
Issue
11
Pages
1321–1328
Identifiers
PMID: 9774631
Source
Medline
License
Unknown

Abstract

The polyamines (PAs) are ubiquitous polycationic metabolites in eukaryotic and prokaryotic cells and are believed to be intimately involved in the regulation of DNA, RNA, and protein biosynthesis, the exact function of which remains unclear, mainly because of a lack of knowledge of PA subcellular localization. In this study, using immunoelectron microscopy, we have demonstrated that PAs are predominantly located on free and attached ribosomes of the rough endoplasmic reticulum in the neurons of the lateral reticular nucleus of rat medulla oblongata. The nuclei, axons, and nerve endings were devoid of PA. This suggests that PAs are one of the components of biologically active ribosomes, being closely involved in the translation processes of protein biosynthesis.

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