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Immunodetection of tubulin carboxypeptidase activity on nitrocellulose membrane after gel electrophoresis and blotting.

Authors
  • Smania, A M
  • Argaraña, C E
  • Weizetfel, J C
  • Barra, H S
Type
Published Article
Journal
Biochemistry international
Publication Date
Dec 01, 1992
Volume
28
Issue
5
Pages
921–928
Identifiers
PMID: 1288502
Source
Medline
License
Unknown

Abstract

It was found that the detyrosination of tyrosinated tubulin by tubulin carboxypeptidase can occur when both the enzyme and the substrate are adsorbed on nitrocellulose. This, and the use of a specific antibody that recognizes detyrosinated tubulin allowed us to localize tubulin carboxypeptidase on a nitrocellulose membrane after agarose gel electrophoresis and blotting. The method was also extended to detect pancreatic carboxypeptidase A.

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