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Immunocytochemical localization of M2 muscarinic receptors in rat ventricles with anti-peptide antibodies.

Authors
  • Fu, M
  • Schulze, W
  • Wolf, W P
  • Hjalmarson, A
  • Hoebeke, J
Type
Published Article
Journal
The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society
Publication Date
Mar 01, 1994
Volume
42
Issue
3
Pages
337–343
Identifiers
PMID: 8308250
Source
Medline
License
Unknown

Abstract

We produced antibodies against a synthetic peptide corresponding to amino acids 168-192 of the second extracellular loop of the M2 human muscarinic receptor in rabbits. In immunoblot, affinity-purified antibodies specifically recognized a major band of rat ventricular muscarinic receptor protein with a molecular weight of about 80 KD. This recognition could be blocked by pre-incubation with peptide. Moreover, with both light (LM) and electron microscopic (EM) immunocytochemistry techniques, muscarinic receptors were detected on sarcolemma and T-tubules of rat cardiomyocytes. In addition, immunoreactions were localized in membranes of capillaries. Likewise, these reactivities were abolished by pre-incubation with peptide. These results suggest that the antibodies against the second extracellular loop of human M2 muscarinic receptor could specifically recognize rat ventricular muscarinic receptor protein and could be a powerful tool to study the fate of this receptor under different pathological or physiological conditions.

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