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Immunocytochemical characterization of viruses and antigenic macromolecules in viral vaccines.

Authors
  • Nguyen, V M
  • Hoang, T N
  • Huynh, T P
  • Nguyen, T V
  • Nguyen, K G
  • Nguyen, M L
  • Nguyen, T T
  • Dunia, I
  • Cohen, J
  • Benedetti, E L
Type
Published Article
Journal
Comptes rendus de l'Académie des sciences. Série III, Sciences de la vie
Publication Date
Sep 01, 2001
Volume
324
Issue
9
Pages
815–827
Identifiers
PMID: 11558328
Source
Medline
License
Unknown

Abstract

Gold immunolabeling combined with negative staining (GINS) provides a valuable immunocytochemical approach that allows a direct ultrastructural definition of all viral vaccine constituents that share common antigenic features with pathogenic viral particles. These results have implications for the development of viral vaccines since it has been demonstrated that incomplete viral particles such as natural empty capsides and Rotavirus-like particles lacking the infective genome are potential candidates for the production of neutralizing antibodies. Furthermore comparative results of the application of GINS to either inactivated vaccines or unfixed samples provide direct evidence that even after inactivation specific antigenic sites are still available for gold immunolabeling.

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