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Immunochemical detection and characterisation of osteocalcin from moa bone.

Authors
  • Huq, N L
  • Rambaud, S M
  • Teh, L C
  • Davies, A D
  • McCulloch, B
  • Trotter, M M
  • Chapman, G E
Type
Published Article
Journal
Biochemical and biophysical research communications
Publication Date
Jun 28, 1985
Volume
129
Issue
3
Pages
714–720
Identifiers
PMID: 3874623
Source
Medline
License
Unknown

Abstract

Osteocalcin (the 6,000 dalton Mr gamma-carboxyglutamate-containing protein of bone) has been detected in acid extracts of bones of the extinct class of New Zealand ratite birds, the moas, using a radioimmunoassay for sheep osteocalcin. The immunoreactive osteocalcin of the extracts of two of these bones (the fibulae from two specimens of Pachyornis elephantopus found in South Island swamps) has been fractionated by gel filtration chromatography and reversed-phase high performance liquid chromatography, and behaves in a manner characteristic of osteocalcin from modern bones. Carbon-14 dating of bones and gizzard contents found in association with these specimens indicates approximate ages of 3,600 and 7,400 years respectively.

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