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An immune signalling kinase AaMEK3 from mosquitoes: cDNA cloning and characterization.

Authors
Type
Published Article
Journal
Insect Molecular Biology
0962-1075
Publisher
Wiley Blackwell (Blackwell Publishing)
Publication Date
Volume
12
Issue
6
Pages
595–603
Identifiers
PMID: 14986920
Source
Medline

Abstract

In mammals, the mitogen-activated protein (MAP) kinase pathway is one of the four major signalling systems that respond to stress and inflammatory stimuli. A full-length cDNA corresponding to Aedes aegypti MAP kinase kinase 3 (AaMEK3) was cloned and sequenced. It is 1.7 kb and contains an open reading frame of 334 amino acids and eleven conserved kinase domains, including signatures of a putative serine/threonine kinase active site and an ATP binding site. The messenger (mRNA) and protein expression levels of AaMEK3 are enhanced post bacterial inoculation. The in vitro kinase activity assay reveals that (1) AaMEK3 is not autophosphorylated but can phosphorylate myelin basic protein successfully, and (2) it is slightly enhanced by lipopolysaccharide stimulation. This suggests that AaMEK3 may be involved in mosquito immune signalling.

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