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Immune response to phosphorylcholine. I.V. Comparison of homologous and isologous anti-idiotypic antibody.

Authors
  • Köhler, H
  • Richardson, R C
  • Smyk, S
Type
Published Article
Journal
Journal of immunology (Baltimore, Md. : 1950)
Publication Date
Jan 01, 1978
Volume
120
Issue
1
Pages
233–238
Identifiers
PMID: 75225
Source
Medline
License
Unknown

Abstract

Homologous and isologous anti-idiotypic antibodies against the PC-binding BALB/c myeloma protein HOPC-8 were raised in A/He and BALB/c mice, respectively. Isologous and homologous anti-HOPC-8 serum suppressed the response to PC in vitro specifically. The antibodies were purified by using HOPC-8 immunoabsorbent. Purified isologous and homologous anti-idiotypic antibody were labeled with 125I and compared for Ig class composition and idiotype-binding specificity. Both kinds of anti-idiotypic antibodies were predominantly IgG1, were highly specific for the HOPC-8 idiotype, and had a smiliar affinities for the PC-binding site. These findings demonstrate that anti-HOPC-8 antibodies raised in A/He and BALB/c mice are very similar in their biologic and immunochemical poperties.

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