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Immortalization of human myogenic progenitor cell clone retaining multipotentiality.

Authors
  • Hashimoto, Naohiro
  • Kiyono, Tohru
  • Wada, Michiko R
  • Shimizu, Shirabe
  • Yasumoto, Shigeru
  • Inagawa, Masayo
Type
Published Article
Journal
Biochemical and biophysical research communications
Publication Date
Oct 06, 2006
Volume
348
Issue
4
Pages
1383–1388
Identifiers
PMID: 16919240
Source
Medline
License
Unknown

Abstract

Human myogenic cells have limited ability to proliferate in culture. Although forced expression of telomerase can immortalize some cell types, telomerase alone delays senescence of human primary cultured myogenic cells, but fails to immortalize them. In contrast, constitutive expression of both telomerase and the E7 gene from human papillomavirus type 16 immortalizes primary human myogenic cells. We have established an immortalized primary human myogenic cell line preserving multipotentiality by ectopic expression of telomerase and E7. The immortalized human myogenic cells exhibit the phenotypic characteristics of their primary parent, including an ability to undergo myogenic, osteogenic, and adipogenic terminal differentiation under appropriate culture conditions. The immortalized cells will be useful for both basic and applied studies aimed at human muscle disorders. Furthermore, immortalization by transduction of telomerase and E7 represents a useful method by which to expand human myogenic cells in vitro without compromising their ability to differentiate.

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