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Immobilization of recognition elements on a self-assembled monolayers bio-platform

Authors
  • Reyes Cuellar, Julia C.
Publication Date
Jan 01, 2017
Source
DIALNET
Keywords
Language
English
License
Unknown
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Abstract

Tailored materials formed by spontaneous two-dimensional arrangement of 3-aminopropyltriethoxysilane self-assembled monolayer on glass (amino-functionalized glass) has been exploited to attach biomolecules in well-organized structures useful in biosensing. Succinimidyl ester of both unpolymerized diacetylene liposome (NHS-DA-liposome) layer and PEGylated biotin (Bt-PEG-NHS) matrix were covalently bonded to the amino-functionalized glass by the NHS linker, and exposed to either Tyrosinase (Ty) or Streptavidin (SAV) solution. The interaction between Ty and polymerized NHS-PDA-liposome transformed the planarity of the PDA backbone, and a blue-to-red transition occurred; Bt-PEGattached to the fluorescent-SAV by bioaffinity. Sensing capability of bioplatform systems was evaluated by Uv-vis spectroscopy or fluorescence microscopy. Biomolecule functionalized SAMs retained the recognition potential of colorimetric Ty-PDA-liposome after biological interaction, and also facilitated the fabrication of a protein-resistant matrix with a particular affinity property. This surface chemistry is accessible to depositing proteins on both SAM-coated glass surface, and tethered to SAM, resulting in optical bioplatform arrays. / Los materiales funcionalizados poradsorción sobre capas autoensambladas de 3-aminopropiltrietoxisilano (APTS) en vidrio (vidrio amino-funcionalizado) sirven para inmovilizar biomoléculasen estructuras usadas para biosensores. Liposomas de polidiacetileno (lip-PDA-NHS) y Biotin PEGiladocon NHS se inmovilizaron aprovechando el éster de succinimidilo; y sirvieron para reconocer Tirosinasa o streptavidina (SAV).Debido a la interacción liposoma-PDA-Ty, ocurrió un cambio en la planaridad de la cadena polimérica PDA, percibido como una transición de azul-a-rojo; mientras que, la biotina inmovilizada interactúo con SAVpor afinidad biológica. La capacidad de detección del sistemase evaluó por espectroscopia UV-vis o por microscopia de fluorescencia. Las capas de APTS funcionalizadas con biomoléculasretuvieron la capacidad de reconocimiento colorimétrico después de la interacción biológica,permitiendo la fabricación de una matriz resistente a proteínas con una propiedad de bioafinidad. Esta química de superficies es simple y accesible parala modificación de substratos de vidrio, útiles en arreglos de bioplataformas ópticas.

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