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Immobilization of β-glucosidase on bifunctional periodic mesoporous organosilicas

Authors
  • Guan, Liye1
  • Di, Bin1, 2
  • Su, Mengxiang1
  • Qian, Jie1
  • 1 China Pharmaceutical University, Department of Pharmaceutical Analysis, Nanjing, 210009, China , Nanjing (China)
  • 2 China Pharmaceutical University, Key Laboratory of Drug Quality Control and Pharmacovigilance, Ministry of Education, Nanjing, 210009, China , Nanjing (China)
Type
Published Article
Journal
Biotechnology Letters
Publisher
Springer-Verlag
Publication Date
Apr 17, 2013
Volume
35
Issue
8
Pages
1323–1330
Identifiers
DOI: 10.1007/s10529-013-1208-4
Source
Springer Nature
Keywords
License
Yellow

Abstract

The aminopropyl-functionalized ethane-bridged bifunctional periodic mesoporous organosilicas (APEPMOs) were synthesized by the co-condensation of 1,2-bis (triethoxysilyl) ethane and 3-aminopropyltriethoxysilane in the presence of cationic surfactants octadecyltrimethylammonium chloride in basic medium. The pores of the APEPMOs were expanded with N,N-dimethyldecylamine and the pore-expanded materials were utilized as supports for β-glucosidase immobilization. A high enzyme loading of 120 mg per g support was achieved in 18 h, and 95.5 % of enzymatic activity was retained. β-Glucosidases were strongly immobilized on APEPMOs with only 5 % desorption in the washing step with buffer solution. The immobilized enzyme had 75 % activity after 20 batch reactions and had improved thermal stability, relative to the free enzyme. These results demonstrate that APEPMOs would be promising supports for β-glucosidases immobilization.

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